Anti-nociceptive Potential of Olmuv™ and its Safety Study through Skin Irritation Test

Anti-nociceptive Potential of Olmuv™ and its Safety Study through Skin Irritation Test

(Advancements in Homeopathic Research, Vol. 6 No.2, May 2021-July 2021, Date of Publication 2021/05/1, Pages no. 18-23)

Rohit Bhardwaj1, Amit Kumar Mittal2, Nitesh Sharma3, Swati Madan4,

Harsha Kharkwal5,  Satyendra Kumar Rajput6, Ramachandran Valavan7

1M Pharm, Assistant Professor, Department of Pharmaceutical Sciences, Gurukula Kangri, Haridwar, Uttarakhand

2PhD, Amity Institute of Pharmacy, Amity University, Noida, U.P.

3BHMS, Scientific Officer, Dr. Willmar Schwabe India Pvt. Ltd., U.P.

4PhD, Assistant Director, Amity Institute of Indian System of Medicine, Amity University, Noida, U.P.

5PhD, Director, Amity Institute of Phytomedicine and Phytochemistry, Amity University, Noida, U.P.

6Professor & Head, Department of Pharmaceutical Sciences, Gurukula Kangri Haridwar, Uttarakhand

7MD (Hom), MBA, Head – Scientific & Medical Affairs, Dr. Willmar Schwabe India Pvt. Ltd., Noida, U.P.

Address for correspondence:

Dr. R. Valavan

Dr. Willmar Schwabe India Pvt. Ltd.,

A 36, Sector 60, Noida, Uttar Pradesh, Pin – 201304.

Email: dr.valavan@schwabeindia.com,

Ph.:  9312309186

Abstract

Introduction: Topical applications in the pain management play an important role. A formulation Olmuv™ developed with herbs used in homeopathy is targeted to treat pains of different origins. Though its ingredients are well-established traditionally as well as scientifically, there was no study conducted on this formulation. Hence this study was conducted with the objective to find out the anti-nociceptive effects and safety (skin irritation potential) of OlmuvTM.

Materials and methods: Anti-nociceptive effect of Olmuv™ was conducted in Swiss albino mice. Animals were divided into 4 groups viz. vehicle control, disease control, standard therapy and treatment dose (6ml/kg). Pain was induced by the administration of carrageenan 0.1ml (1% solution) in paw joint region of animals. Eddy’s hot plate and tail flick method of hot water and cold-water assay were used to evaluate by the response time in seconds. Statistical analysis was accomplished using one-way analysis of variance (ANOVA) considering p value <=0.05 as significant. Skin irritation study was conducted by applying approximately 0.04 ml of test product on the marked test site using a brush in healthy human volunteers. This was un-occluded till it dried off, post which the test site was occluded. Filter papers dipped in 1% Sodium Lauryl Sulphate (SLS) solution and 0.9% Isotonic Saline Solution were filled in another well of patch test chambers and both were applied occlusively on the back of participants by Clinical Research Associates at Hour 0. Evaluation was done after 48 hours with scoring for erythema and oedema from 1 to 4.