Rohit Bhardwaj1, Amit Kumar Mittal2, Nitesh Sharma3, Swati Madan4,
Harsha Kharkhwal5, Satyendra Kumar Rajput6, Ramachandran Valavan7*
1B Pharm, M Pharm Scholar, Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh, India. Pin – 201303
2PhD, Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh, India. Pin – 201303
3BHMS, Scientific Officer, Dr. Willmar Schwabe India Pvt. Ltd., A 36, Sector 60, Noida, Uttar Pradesh, India. Pin – 201304
4PhD, Assistant Director, Amity Institute of Indian System of Medicine, Amity University, Noida, Uttar Pradesh, India. Pin – 201303
5PhD, Director, Amity Institute of Phytomedicine and Phytochemistry, Amity University, Noida, Uttar Pradesh, India. Pin – 201303
6PhD, Director, Amity Institute of Indian System of Medicine, Amity University, Noida, Uttar Pradesh, India. Pin – 201303
7MD (Hom), Head – Scientific & Medical Affairs,
Dr. Willmar Schwabe India Pvt. Ltd., A 36, Sector 60, Noida, Uttar Pradesh, India. Pin – 201304
*Address for correspondence –
Dr. R. Valavan,
Head – Scientific & Medical Affairs, Dr. Willmar Schwabe India Pvt. Ltd., A 36, Sector 60, Noida, Uttar Pradesh, India. Pin – 201304.
Email: dr.valavan@schwabeindia.com,
Phone: 9312309186
Abstract
Introduction: Spine-related problems are continuously increasing due to current lifestyle, environmental and genetic factors. Lifestyle factors like prolonged sitting, lack of exercise and wrong posturefurther aggravate these problems. A homoeopathic formulation Rück-Pain™ was developed to treat the symptoms arising from spinal disorders and surrounding regions. Its ingredients are covered by homoeopathic pharmacopoeia (s) and authoritative literature.
Objective: The current study aimed to evaluate the anti-inflammatory, anti-nociceptive and toxicity effects of Rück-Pain™ in mice animal model.
Materials and methods: Swiss albino mice were divided into 6 groups viz. vehicle control, disease control, treatment dose 1 (2.96ml/kg), treatment dose 2 (6ml/kg), treatment dose 3 (8.80ml/kg) and standard treatment. Spondylitis was induced by the administration of Sodium thioglycolate 0.1ml (1% solution) at neck joint region of mice. Anti-inflammatory effect was evaluated by measuring erythrocyte sedimentation rate (ESR) and area of inflammation in volume in the neck region by Vernier caliper. Anti-nociceptive effect was evaluated using tail-flick assay. Histological changes in liver and spleen were assessed to find out the toxicity by the treatment groups. Statistical analysis was done using one-way analysis of variance (ANOVA) considering P=<0.05 as significant.
Results : Results of ESR in the groups of treatment doses 1, 2 and 3 were found 1.75 mm/h, 1. 62 mm/h and 1.50 mm/h respectively against 1.95 mm/h in disease control group and 1.55 in the standard treatment group. Area of inflammation in the groups of treatment doses 1, 2 and 3 were found to be reduced to 1.65 cm2, 1.57 cm2 and 1.53 cm2 respectively against 1.9 cm2 in disease control group and 1.55 cm2in the standard treatment group. Nociceptive effect in the groups of treatment doses 1, 2 and 3 were found to be reduced to 9 seconds, 10 seconds and 10 seconds respectively against 7 seconds in disease control group and 10.5 seconds in the standard treatment group. Histological assessment showed there were no significantly observed cellular changes.
Conclusion : In the current study, homoeopathic formulation Rück-PainTM exhibited anti-inflammatory and nociceptive activities against spondylitis in animal spine system. It has effectively reduced the inflammation and pain due to spondylitis. Further, the formulation was found to be non-toxic to liver and spleen of mice. It can be a safe and effective therapy for the management of the spondylitis and associated spinal and back disorders.